Laboratory of Viral Immunity and Pathogenesis (LVIP)

The Viral Immunity and Pathogenesis Laboratory is a ~2,400 sq ft BSL-2 laboratory with dedicated tissue culture space and molecular biology space. Major equipment includes:

• Class IIA biosafety cabinets (5)
• CO₂ incubators (5; one equipped with refrigeration for 28°C culture)
• Refrigerated centrifuges (2)
• Liquid nitrogen storage tanks (6; combined capacity >100,000 vials)
• Ultralow temperature freezers (5; combined capacity >100,000 vials)
• Inverted microscope
• EVOSfl fluorescence microscope (includes GFP, RFP, DAPI, and Cy5 cubes, 100× oil immersion objective)
• Nexcelom Cellometer T4 and Mini automated cell counters
• PCR hood
• QIAcube (automated nucleic acid isolation)
• QIAgility (liquid handler/automated PCR assay setup)
• Bio-Rad dual 48-well PCR machine
• Bio-Rad CFX96 real-time PCR machine
• Bio-Rad Chemi-Doc XRS imager
• Envision multilabel plate reader (includes filters for fluorescence, luminescence, and AlphaScreen)
• BioTek Cytation3 cell imaging plate reader
• Bio-Rad Bioplex 200 Luminex
• MACSQuant-10 flow cytometer (3 lasers, 8-color capacity)
• BD LSRII flow cytometer (4 lasers, 12-color capacity)
• CTL S5 UV ELISpot reader and analyzer
• Miltenyi AutoMACS
• 10x Genomics Chromium Controller