{"id":243,"date":"2013-09-23T10:38:57","date_gmt":"2013-09-23T14:38:57","guid":{"rendered":"https:\/\/web.uri.edu\/pbl\/?page_id=243"},"modified":"2026-03-11T09:49:40","modified_gmt":"2026-03-11T13:49:40","slug":"plant-transformation","status":"publish","type":"page","link":"https:\/\/web.uri.edu\/pbl\/plant-transformation\/","title":{"rendered":"Plant Transformation"},"content":{"rendered":"\n<p>We operate comprehensive platforms for stable integrative transformation, CRISPR-based genome editing, and regeneration of engineered events across diverse plant species. Capabilities include method and technology development for customized collaborative projects; i.e. exploratory projects, new species or cultivar, new protocols, , vector preparation, delivery optimization, and molecular validation. Greenhouse and growth chambers support full lifecycle evaluation.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Capabilities<\/strong><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Agrobacterium transformation (dicots &amp; monocots)<\/li>\n\n\n\n<li>Biolistic transformation (including chloroplast targets)<\/li>\n\n\n\n<li>Protoplast transfection and transient expression<\/li>\n\n\n\n<li>CRISPR (Cas9\/Cas12a), multiplex editing, base\/prime editing workflows<\/li>\n\n\n\n<li>PCR\/qPCR\/RT-PCR, genotyping, Sanger\/amplicon validation<\/li>\n\n\n\n<li>GFP and brightfield imaging; phenotype assessment<\/li>\n\n\n\n<li>Access to genomics &amp; DNA sequencing capabilities via URI core<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Subpages<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Collaborations: how to partner, scopes, timelines<\/li>\n\n\n\n<li>Training: workshops and hands-on formats<\/li>\n\n\n\n<li>Contracts: How to set up collaborative contracts, fee-for-service, MTAs, IP, compliance<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><a class=\"cl-button   prominent\" href=\"#\" title=\"\">Start a Collaboration <\/a><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><a class=\"cl-button   prominent\" href=\"#\" title=\"\">Schedule TraininG<\/a><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><a class=\"cl-button   prominent\" href=\"#\" title=\"\">Request a Quote<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>We operate comprehensive platforms for stable integrative transformation, CRISPR-based genome editing, and regeneration of engineered events across diverse plant species. Capabilities include method and technology development for customized collaborative projects; i.e. exploratory projects, new species or cultivar, new protocols, , vector preparation, delivery optimization, and molecular validation. Greenhouse and growth chambers support full lifecycle evaluation. [&hellip;]<\/p>\n","protected":false},"author":581,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-243","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/243","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/users\/581"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/comments?post=243"}],"version-history":[{"count":1,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/243\/revisions"}],"predecessor-version":[{"id":702,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/243\/revisions\/702"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/media?parent=243"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}