{"id":676,"date":"2026-03-11T09:32:26","date_gmt":"2026-03-11T13:32:26","guid":{"rendered":"https:\/\/web.uri.edu\/pbl\/?page_id=676"},"modified":"2026-03-11T09:32:26","modified_gmt":"2026-03-11T13:32:26","slug":"research-2","status":"publish","type":"page","link":"https:\/\/web.uri.edu\/pbl\/research-2\/","title":{"rendered":"Research"},"content":{"rendered":"\n

Our research includes plant transformation technology development, genome engineering, and functional genomics, with applications in crop improvement, stress resilience, and metabolic pathway engineering. We bridge fundamental discovery and translational outcomes through robust tissue culture pipelines and molecular validation.<\/p>\n\n\n\n

Focus Areas<\/strong>
<\/h2>\n\n\n\n
\n

Plant Transformation and Plant Regeneration <\/h2>

Multi-species protocols; Maize, rice sorghum and switchgrass.- callus induction; somatic embryogenesis; rooting and acclimatization.<\/p><\/div><\/div><\/a><\/div><\/div>\n\n\n\n

Genome Editing and Trait Engineering <\/h2>

CRISPR\u2013Cas systems (Cas9, Cas12a),
base\/prime editing, multiplex gRNA design, RNP & plasmid delivery.<\/p><\/div><\/div><\/a><\/div><\/div>\n<\/div>\n\n\n\n

\n

Functional Genomics <\/h2>

Gene knockout\/knock-in; expression and promoter analysis; pathway dissection.<\/p><\/div><\/div><\/a><\/div><\/div>\n\n\n\n

Molecular Characterization <\/h2>

DNA\/RNA extraction, PCR\/qPCR\/RT-PCR, imaging and phenotyping.<\/p><\/div><\/div><\/a><\/div><\/div>\n<\/div>\n\n\n\n

\n

Growth and Phenotyping <\/h2>

Greenhouse and Controlled environments<\/p><\/div><\/div><\/a><\/div><\/div>\n<\/div>\n\n\n\n

Methods and Platforms<\/strong><\/h2>\n\n\n\n

Agrobacterium-mediated transformation<\/strong><\/p>\n\n\n\n

    \n
  • Sorghum<\/strong><\/li>\n\n\n\n
  • Maize<\/strong><\/li>\n\n\n\n
  • Rice<\/strong><\/li>\n<\/ul>\n\n\n\n

    Biolistic gene delivery (PDS-1000\/He, Bio-Rad)<\/strong><\/p>\n\n\n\n

    Microscopy -Brightfield, GFP fluorescence (FLM), Scanning electron microscopy (SEM)<\/strong><\/p>\n\n\n\n

    Outcomes & Impact<\/strong><\/h2>\n\n\n\n

    Bioenergy and Crop Trait Development (yield, nutrition, stress tolerance)<\/p>\n\n\n\n

    Synthetic biology and biomanufacturing in plants<\/p>\n\n\n\n

    Marker-free precision breeding<\/p>\n","protected":false},"excerpt":{"rendered":"

    Our research includes plant transformation technology development, genome engineering, and functional genomics, with applications in crop improvement, stress resilience, and metabolic pathway engineering. We bridge fundamental discovery and translational outcomes through robust tissue culture pipelines and molecular validation. Focus Areas Methods and Platforms Agrobacterium-mediated transformation Biolistic gene delivery (PDS-1000\/He, Bio-Rad) Microscopy -Brightfield, GFP fluorescence (FLM), […]<\/p>\n","protected":false},"author":5144,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-676","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/676","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/users\/5144"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/comments?post=676"}],"version-history":[{"count":1,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/676\/revisions"}],"predecessor-version":[{"id":678,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/676\/revisions\/678"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/media?parent=676"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}