The PBL comprises >4,900 sq ft of laboratory space with a 1,200 sq ft centralized transgenics core and a 400 sq ft media lab, plus access to greenhouses, field sites, and a university genomics facility. Spaces support plant tissue culture, transformation, genome editing, molecular analysis, and imaging.<\/p>\n\n\n\n
Tissue Culture & Core Spaces<\/p>\n\n\n\n
Twenty laminar flow hoods for plant tissue culture<\/p>\n\n\n\n
Eight student six-foot laminar flow hoods (teaching\/demo)<\/p>\n\n\n\n
Media preparation lab (400 sq ft) with sterilization and prep equipment<\/p>\n\n\n\n
Incubators (various sizes), orbital shakers, water baths<\/p>\n\n\n\n
Autoclaves (on-site and nearby), fume hoods<\/p>\n\n\n\n
Growth rooms and controlled plant growth chambers<\/p>\n\n\n\n
URI greenhouse and field space access for grow-out and analysis<\/p>\n\n\n\n
Molecular Biology & Analysis<\/p>\n\n\n\n
Super-speed and ultracentrifuge, microfuges, benchtop centrifuges<\/p>\n\n\n\n
Vertical & horizontal electrophoresis units; power supplies<\/p>\n\n\n\n
Multiple thermocyclers for PCR, qPCR, and RT-PCR<\/p>\n\n\n\n
DNA\/RNA extraction; pH meters, speed-vac, vacuum ovens<\/p>\n\n\n\n
Spectrophotometers; fluorometer for DNA quantitation<\/p>\n\n\n\n
Scintillation counter; lyophilizer<\/p>\n\n\n\n
Refrigerators\/freezers (including two \u221280\u202f\u00b0C freezers); 4\u202f\u00b0C reach-in cooler<\/p>\n\n\n\n
Gene Transfer Platforms<\/p>\n\n\n\n
Biolistics: PDS-1000\/He Particle Delivery System (Bio-Rad)<\/p>\n\n\n\n
Agrobacterium-mediated transformation systems<\/p>\n\n\n\n
Electroporation: GenePulser II (Bio-Rad)<\/p>\n\n\n\n
Microinjection and other delivery methods<\/p>\n\n\n\n
Imaging & Documentation<\/p>\n\n\n\n
Zeiss Discovery V2 GFP fluorescence microscope (assorted lenses)<\/p>\n\n\n\n
Light microscopy: two Olympus dissecting scopes with CCD cameras<\/p>\n\n\n\n
Kodak EDAS Gel Documentation System, UV transilluminator, light box<\/p>\n\n\n\n
Slab gel dryer; phosphorimager with analysis software<\/p>\n\n\n\n
Photography: two Nikon high-definition digital cameras (wide-angle & macro lenses), light tables, tripods<\/p>\n\n\n\n
High-definition digital video camera with blue screen<\/p>\n\n\n\n
Image analysis workstation (Dell; scanner\/video inputs)<\/p>\n\n\n\n
Computing & Networking<\/p>\n\n\n\n
Mixed Windows and macOS workstations; iPads (Mac)<\/p>\n\n\n\n
Networked laser printers (color & B\/W) and a Xerox document center<\/p>\n\n\n\n
Direct connection to university compute resources and campus networking<\/p>\n\n\n\n
Video conferencing support (e.g., Teams\/Zoom\/Skype); wireless networking<\/p>\n\n\n\n
Additional Access<\/p>\n\n\n\n
URI Genomics & DNA Sequencing capabilities (university facility)<\/p>\n\n\n\n
Photographic darkroom access<\/p>\n\n\n\n
Ice machines; autoclaves (nearby)<\/p>\n\n\n\n
Conference room; teaching laboratory space<\/p>\n\n\n\n
Administrative and purchasing support via CBLS and on-site offices<\/p>\n\n\n\n
Compliance & Scope Notes<\/p>\n\n\n\n
No clinical or animal research is conducted in the PBL.<\/p>\n\n\n\n
Equipment and availability may change; please contact us for current status or scheduling.<\/p>\n","protected":false},"excerpt":{"rendered":"
The PBL comprises >4,900 sq ft of laboratory space with a 1,200 sq ft centralized transgenics core and a 400 sq ft media lab, plus access to greenhouses, field sites, and a university genomics facility. Spaces support plant tissue culture, transformation, genome editing, molecular analysis, and imaging. Tissue Culture & Core Spaces Twenty laminar flow […]<\/p>\n","protected":false},"author":5144,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-688","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/688","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/users\/5144"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/comments?post=688"}],"version-history":[{"count":2,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/688\/revisions"}],"predecessor-version":[{"id":708,"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/pages\/688\/revisions\/708"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/pbl\/wp-json\/wp\/v2\/media?parent=688"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}