{"id":3919,"date":"2021-08-10T08:49:23","date_gmt":"2021-08-10T12:49:23","guid":{"rendered":"https:\/\/web.uri.edu\/pdi\/?page_id=3919"},"modified":"2023-07-20T08:51:24","modified_gmt":"2023-07-20T12:51:24","slug":"cell-harvesting","status":"publish","type":"page","link":"https:\/\/web.uri.edu\/pdi\/training\/cell-harvesting\/","title":{"rendered":"Cell Harvesting"},"content":{"rendered":"

Cell Harvesting<\/h1>

Participants will learn about the theories and practices of initial cell harvest and separation<\/p><\/div><\/div>

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Apply site-wide<\/div><\/div><\/div><\/div><\/div><\/div><\/section>\n

Want to know more?<\/h1>

Email pdi@etal.uri.edu<\/a>
\nor call 401.874.3114<\/b>
\n<\/p><\/div><\/div>\n

Critical parameters and performance indicators provide confirmation of technicians\u2019 proficiencies in cell harvest and initial cell separation methodologies.<\/span><\/span><\/p>\n

Participants gain knowledge of <\/span>the theories and practices of initial cell harvest and separation. Critical parameters and performance indicators provide confirmation of technicians\u2019 proficiencies in cell harvest and initial cell separation methodologies.<\/span><\/span> <\/span><\/p>\n

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Objectives<\/h1>

<\/p>\n

    \n
  • Attain knowledge about the theories and practices associated with initial cell harvest and separation techniques<\/li>\n
  • Perform hands-on techniques associated with acid precipitation of suspension cell culture, centrifugation and harvest of supernatant, and depth filtration of supernatant and preparation for downstream processing.<\/li>\n
  • Understand critical process parameters and performance indicators required for optimized cell harvest (yield and retention) and cell separation.<\/li>\n<\/ul>\n

    <\/p><\/article><\/div><\/section>\n

     <\/p>\n

    Who should attend this course?<\/h1>\n

    Course work and laboratory exercises are designed for individuals working in the <\/span>b<\/span>iomanufacturing industry including roles such as Upstream and Downstream Technicians, Quality Assurance, Quality Control, Bioprocess Engineers, Facilities<\/span>,<\/span> and Plant Operations<\/span>.<\/span><\/span> <\/span><\/p>\n

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    Delivery<\/h1>

    In-person lectures and hands on activities in a simulated GMP facility.        <\/p>\n

      \n
    • Duration: 1 Day<\/li>\n
    • Time: 8:00am to 4:00pm<\/li>\n
    • Location: Kingston, RI<\/li>\n
    • Max Number of Participants: 15<\/li>\n<\/ul>\n

      <\/p><\/article><\/div><\/section>\n

       <\/h3>\n

      Instructors<\/h1>\n\n

      Content Outline<\/h1>\n

      Lecture<\/h2>\n
        \n
      1. \n
          \n
        1. Cell harvest Overview\n
            \n
          • What is cell separation?<\/li>\n
          • Batch and Perfusion\/continuous processes<\/li>\n<\/ul>\n<\/li>\n
          • Methods for separation\n
              \n
            • Precipitation<\/li>\n
            • Theory supporting precipitation methods<\/li>\n
            • Modes of precipitation<\/li>\n
            • Yield and Retention<\/li>\n
            • Process Parameters and Performance Indicators<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n
                \n
              1. Centrifugation\n
                  \n
                • Theory supporting centrifugation methods<\/li>\n
                • Types of centrifuges<\/li>\n
                • Yield and Retention<\/li>\n
                • Process Parameters and Performance Indicators<\/li>\n<\/ul>\n<\/li>\n
                • Filtration\n
                    \n
                  • Theory supporting filtration methods membranes and Types of filtration methods<\/li>\n
                  • Yield and Retention<\/li>\n
                  • Process Parameters and Performance Indicators<\/li>\n<\/ul>\n<\/li>\n
                  • Systems Summary and Challenges<\/li>\n<\/ol>\n

                    Lab Exercises<\/h2>\n
                      \n
                    1. Acid precipitation of suspension cell culture<\/li>\n
                    2. Centrifugation of cell culture and harvest of supernatant<\/li>\n
                    3. Filtration of supernatant and preparation for downstream processes<\/li>\n
                    4. Analysis of filtrate for desired cell product<\/li>\n<\/ol>\n

                       <\/p>\n

                       <\/p>\n","protected":false},"excerpt":{"rendered":"

                          Critical parameters and performance indicators provide confirmation of technicians\u2019 proficiencies in cell harvest and initial cell separation methodologies. Participants gain knowledge of the theories and practices of initial cell harvest and separation. Critical parameters and performance indicators provide confirmation of technicians\u2019 proficiencies in cell harvest and initial cell separation methodologies.    Who should […]<\/p>\n","protected":false},"author":639,"featured_media":0,"parent":2234,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3919","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/pages\/3919","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/users\/639"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/comments?post=3919"}],"version-history":[{"count":5,"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/pages\/3919\/revisions"}],"predecessor-version":[{"id":4563,"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/pages\/3919\/revisions\/4563"}],"up":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/pages\/2234"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/pdi\/wp-json\/wp\/v2\/media?parent=3919"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}