{"id":30513,"date":"2022-02-23T15:39:58","date_gmt":"2022-02-23T20:39:58","guid":{"rendered":"https:\/\/web.uri.edu\/riinbre\/?page_id=30513"},"modified":"2022-02-23T15:39:58","modified_gmt":"2022-02-23T20:39:58","slug":"mic-robotic-sample-prep","status":"publish","type":"page","link":"https:\/\/web.uri.edu\/riinbre\/mic\/mic-services-and-resources\/mic-sequencing-services\/mic-robotic-sample-prep\/","title":{"rendered":"MIC Robotic Sample Prep"},"content":{"rendered":"<div id=\"contentwrap\">\n<div id=\"content\" class=\"list-post\">\n<article id=\"post-308\" class=\"post clearfix cat-5 post-308 type-post status-publish format-standard has-post-thumbnail hentry category-service has-post-title no-post-date has-post-category has-post-tag has-post-comment has-post-author \">\n<div class=\"post-content\">\n<h1 class=\"post-title entry-title\">Robotic Sample Preparation<\/h1>\n<div class=\"entry-content\">\n<h4>Wafergen Apollo 324 NGS Library Preparation System<\/h4>\n<p>The <strong>Apollo 324<\/strong> is a benchtop system that automates next-generation sequence (NGS) library preparation workflows by using bead technology to execute high-performance isolation and purification of nucleic acids (DNA, RNA, plasmid and so on) and proteins.&nbsp; The system automates end-repair, A-tail addition, adapter ligation and final double-bead-based cleanup and size selection.&nbsp; The <strong>Apollo 324<\/strong> enables simultaneous processing of one to eight samples, with batch processing for 32 samples at a time.&nbsp; Up to eight samples can be prepared in approximately four hours, yielding products ready for amplification or direct sequencing, and a batch of 32 libraries can be created in less than eight hours.<\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-431\" src=\"https:\/\/web.uri.edu\/wp-content\/uploads\/sites\/72\/RoboticPrepimage1-1024x768.jpg\" alt=\"\" width=\"491\" height=\"369\">Specifications:<\/strong><\/p>\n<ul>\n<li>Samples per run: 1\u20138 or 32<\/li>\n<li>Throughput\/day: 16 samples: 2 runs of 8 samples | 32 samples: 1 run of 32 samples<\/li>\n<li>Run time: 1\u20138 samples: ~ 3.5 hours | 32 samples: ~ 7 hours<\/li>\n<li>Setup time: 1\u20138 samples: ~ 15 minutes | 32 samples: ~ 1 hour<\/li>\n<li>Temperature control: 2 Peltier-type blocks (4\u201395 \u00b0C), PID regulated<\/li>\n<li>Magnetic capture: In-tip capture<\/li>\n<li>Plate format: 96-well, SBS conforming<\/li>\n<\/ul>\n<figure id=\"attachment_432\" class=\"wp-caption aligncenter\" aria-describedby=\"caption-attachment-432\"><\/figure>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<\/article>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Robotic Sample Preparation Wafergen Apollo 324 NGS Library Preparation System The Apollo 324 is a benchtop system that automates next-generation sequence (NGS) library preparation workflows by using bead technology to execute high-performance isolation and purification of nucleic acids (DNA, RNA, plasmid and so on) and proteins.&nbsp; The system automates end-repair, A-tail addition, adapter ligation and [&hellip;]<\/p>\n","protected":false},"author":3192,"featured_media":0,"parent":29431,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-30513","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/pages\/30513","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/users\/3192"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/comments?post=30513"}],"version-history":[{"count":2,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/pages\/30513\/revisions"}],"predecessor-version":[{"id":31845,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/pages\/30513\/revisions\/31845"}],"up":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/pages\/29431"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/media?parent=30513"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}