{"id":33966,"date":"2023-04-25T10:12:12","date_gmt":"2023-04-25T14:12:12","guid":{"rendered":"https:\/\/web.uri.edu\/riinbre\/?p=33966"},"modified":"2023-09-07T09:11:52","modified_gmt":"2023-09-07T13:11:52","slug":"small_molecule_sep_wdt2023","status":"publish","type":"post","link":"https:\/\/web.uri.edu\/riinbre\/small_molecule_sep_wdt2023\/","title":{"rendered":"Small Molecule Separation (WDT 2023)"},"content":{"rendered":"<h1>RI-INBRE Workforce Development Training&nbsp; (WDT) Summer 2023 Syllabus<\/h1>\n<h2>Module Title: Small Molecule Separation<\/h2>\n<h3>Instructor: Ang Cai, PhD, and Sam Howarth<\/h3>\n<h3>Course Overview<\/h3>\n<p>This 2.5-day RI-INBRE Workforce Development Training (WDT) Module provides an introduction to small molecules separation and chemical structure analysis methods. The module includes lectures on High Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance (NMR), and Liquid Chromatography-Mass spectrometry (LC\/MS) methods, as well as hands-on<br \/>\nlaboratory experience with these techniques. This module is designed for students new to separation and analysis of small molecules and provides sufficient background to pursue further training in related research areas. Upon completion, participants will receive an RI-INBRE Certificate of Completion.<\/p>\n<h2>Learning Outcomes<\/h2>\n<ul>\n<li>Develop kno0wledge of basic chemical separation techniques<\/li>\n<li>Small molecule sample preparation<\/li>\n<li>Proper operation of instrumentation including HPLC, NMR, and LC\/MS<\/li>\n<li>Analysis of chemical structures<\/li>\n<li>Outcomes<\/li>\n<\/ul>\n<h2>Lab Report<\/h2>\n<p>Students will be expected to maintain detailed laboratory notes to include:<\/p>\n<ul>\n<li>Sample preparation<\/li>\n<li>Chemical analysis protocols<\/li>\n<li>Results<\/li>\n<\/ul>\n<h2>Resources Used in this Module:<\/h2>\n<ul>\n<li>Ibuprofen<\/li>\n<li>Caffeine<\/li>\n<li>Aspirin (acetylsalicylic acid)<\/li>\n<li>Benzoic Acid<\/li>\n<li>Acetominophen (paracetamol)<\/li>\n<\/ul>\n<p>The chosen small molecules are readily available from commercial sources. In addition, these<br \/>\nmolecules have different chemical properties that will ensure successful HPLC separation. Each<br \/>\ncompound is also commonly used in pharmaceutical research, making them relevant to a wide<br \/>\nrange of scientific disciplines. Again, the structures of these molecules are well-known, which<br \/>\nwill ensure straightforward structural characterization with NMR, as well as accurate<br \/>\nidentification of their molecular weights using mass spectrometry.<\/p>\n<p>Standard Operating Procedure \u2013 Thermo Ultimate 3000 HPLC<br \/>\nStandard Operating Procedure \u2013 Brucker 300 MHz NMR<br \/>\nStandard Operating Procedure \u2013 ABSciEX TOF 4600 or Thermo LTQ-XL Mass Spectrometer<\/p>\n<h2>Timeline<\/h2>\n\n<table id=\"tablepress-19\" class=\"tablepress tablepress-id-19\">\n<thead>\n<tr class=\"row-1\">\n\t<td class=\"column-1\"><\/td><th class=\"column-2\">Day 1<\/th><th class=\"column-3\">Day 2<\/th><th class=\"column-4\">Day 3<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">9:00 AM-10:00 AM<\/td><td class=\"column-2\">Introduction to HPLC<br \/>\n(Lecture)<\/td><td class=\"column-3\">Introduction to mass<br \/>\nspectrometry and<br \/>\ndemonstration of how to<br \/>\nuse the instrument (Lecture)<\/td><td class=\"column-4\">Conclusion and discussion of<br \/>\nthe experiment results (Open<br \/>\nDiscussion)<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">10:00 AM-11:00 AM<\/td><td class=\"column-2\">Preparation of<br \/>\nsamples for HPLC<br \/>\nanalysis (Hands-On)<\/td><td class=\"column-3\">Analysis of the separated<br \/>\ncompounds from LC with MS<br \/>\n(Hands-On)<\/td><td class=\"column-4\">Conclusion and discussion of<br \/>\nthe experiment results (Open<br \/>\nDiscussion)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">11:00 AM-12:00 PM<\/td><td class=\"column-2\">HPLC Separation<br \/>\n(Hands-On)<\/td><td class=\"column-3\">Analysis of the separated<br \/>\ncompounds from LC with MS<br \/>\n(Hands-On)<\/td><td class=\"column-4\">Conclusion and discussion of<br \/>\nthe experiment results (Open<br \/>\nDiscussion)<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">12:00 - 1:00 PM<\/td><td class=\"column-2\">Break<\/td><td class=\"column-3\">Break<\/td><td class=\"column-4\">Closing Group Lunch<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">1:00 PM-2:00 PM<\/td><td class=\"column-2\">NMR Spectroscopy<br \/>\nintroduction (Lecture)<\/td><td class=\"column-3\">Interpretation of HPLC,<br \/>\nNMR, and MS data to<br \/>\nidentify the two unknown<br \/>\ncompounds (Lecture\/Hands-<br \/>\nOn)<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">2:00 PM-3:00 PM<\/td><td class=\"column-2\">Samples preparation,<br \/>\nstructure acquisition<br \/>\nand Analysis (Hands-<br \/>\non)<\/td><td class=\"column-3\">Interpretation of HPLC,<br \/>\nNMR, and MS data to<br \/>\nidentify the two unknown<br \/>\ncompounds (Lecture\/Hands-<br \/>\nOn)<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\">3:00 PM-4:00 PM<\/td><td class=\"column-2\">Review and discussion<br \/>\nof HPLC and NMR data<br \/>\n(Hands-on)<\/td><td class=\"column-3\">Interpretation of HPLC,<br \/>\nNMR, and MS data to<br \/>\nidentify the two unknown<br \/>\ncompounds (Lecture\/Hands-<br \/>\nOn)<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-19 from cache -->\n","protected":false},"excerpt":{"rendered":"<p>RI-INBRE Workforce Development Training&nbsp; (WDT) Summer 2023 Syllabus Module Title: Small Molecule Separation Instructor: Ang Cai, PhD, and Sam Howarth Course Overview This 2.5-day RI-INBRE Workforce Development Training (WDT) Module provides an introduction to small molecules separation and chemical structure analysis methods. The module includes lectures on High Performance Liquid Chromatography (HPLC), Nuclear Magnetic Resonance [&hellip;]<\/p>\n","protected":false},"author":3192,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[821],"tags":[823,822],"class_list":["post-33966","post","type-post","status-publish","format-standard","hentry","category-wdt2023","tag-hplc","tag-wdt"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/33966","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/users\/3192"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/comments?post=33966"}],"version-history":[{"count":5,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/33966\/revisions"}],"predecessor-version":[{"id":34572,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/33966\/revisions\/34572"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/media?parent=33966"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/categories?post=33966"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/tags?post=33966"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}