Location: CCRI<\/strong> <\/em> This WDT module is designed to teach students the concepts of cell and tissue culture using bacterial and mammalian cells. Students will receive instruction in cell and tissue culture theory and perform hands-on activities to grow and maintain cell lines. This will include learning aseptic technique, working in a biological safety cabinet (BSC), preparing sterile media, using an autoclave, and inoculating cultures. The cultures will be grown in shake flasks, T-flasks, and a 2L bioreactor. The module will be completed over 2.5 days on a Monday, Wednesday, Friday schedule. Participants who complete the module will receive a RI-INBRE Certificate of completion.<\/p>\n Students will be expected to maintain an electronic laboratory notebook in the cohort\u2019s Google classroom. Each student will be given a generic lab template to complete, and students should record an entry for each procedure containing the title of the procedure, date performed and lab partner if applicable. They should write a brief description of the procedure and materials required. They should also record critical steps performed, amounts of reagents used, calculations, and any observations made. Lastly, conclusions should discuss steps that went well and steps that did not go well, and ways that the procedure could be improved to avoid mistakes in the future.<\/p>\n It is recommended that students bring a tablet, iPad, or laptop to make entries in their electronic lab notebook. Lecture slides and laboratory handouts will be provided to students.<\/p>\n Cell and Tissue Culture Foundations Instructor: Scott Warila, Community College of Rhode Island Location: CCRI Session 1: June 8-9, June 11 Session 2: July 15-16, July 18 Course Overview This WDT module is designed to teach students the concepts of cell and tissue culture using bacterial and mammalian cells. Students will receive instruction in […]<\/p>\n","protected":false},"author":3192,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[1],"tags":[],"class_list":["post-36030","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/36030","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/users\/3192"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/comments?post=36030"}],"version-history":[{"count":5,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/36030\/revisions"}],"predecessor-version":[{"id":37889,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/36030\/revisions\/37889"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/media?parent=36030"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/categories?post=36030"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/tags?post=36030"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nSession 1: June 8-9, June 11
\nSession 2: July 15-16, July 18<\/p>\nCourse Overview<\/h3>\n
Learning Outcomes<\/h2>\n
\n
Electronic Laboratory Notebook<\/h2>\n
Additional Information:<\/h2>\n
Proposed Timeline (subject to change)<\/h2>\n\n
\n\n
\n\t \n<\/td> Monday<\/th> Wednesday<\/th> Friday<\/th>\n<\/tr>\n<\/thead>\n \n\t 9:00 AM-10:00 AM<\/td> Introduction to
\ncourse\/Overview of bacterial
\ncell culture and bacterial
\ntransformation (lecture)<\/td>Intro to Bioreactors
\n(lecture) Introduction to the 2L
\nbioreactor and preparation for
\nsterilization (Demo and hands-
\non)<\/td>Observation of E. coli liquid
\ncultures from Day 2
\nInoculation and operation of
\nthe 2L bioreactor (demo and
\nhands-on)<\/td>\n<\/tr>\n\n\t 10:00 AM-11:00 AM<\/td> Preparing LB agar for
\nselection of transformed E.
\ncoli (Demo and hands-on)<\/td>Calibration of 2L bioreactor DO
\nand pH probes and sterilization
\nof the bioreactor by autoclave
\n(hands-on)<\/td>Observation of student
\nprepared CHO cells from Days
\n1 and 2. Cryopreservation of
\nCHO cells (hands-on)<\/td>\n<\/tr>\n\n\t 11:00 AM-12:00 PM<\/td> Transformation of E. coli with
\nGFP plasmid (hands-on)<\/td>Examination of LB agar plates
\nfrom Day 1. Selection of GFP
\nproducing colonies to inoculate
\nLB broth (hands-on)<\/td>STEM cell culture with focus
\non myocardiocytes and tissue
\nregeneration (discussion and
\ndemo)<\/td>\n<\/tr>\n\n\t 12:00 - 1:00 PM<\/td> Break<\/td> Break<\/td> Closing remarks
\nWDT Survey
\nCertificate Distribution<\/td>\n<\/tr>\n\n\t 1:00 PM-2:00 PM<\/td> Introduction to the CO2
\nincubator, BSC, aseptic
\ntechnique, and preparing
\nCHO cell media (hands-on)<\/td>Examination of instructor
\nprepared CHO cell cultures
\nusing Floid cell imager. Perform
\na viable cell count and
\nglucose\/lactate assay.<\/td><\/td>\n<\/tr>\n \n\t 2:00 PM-3:00 PM<\/td> Inoculating CHO cell media
\nin T-flasks and plates from
\ncryovial and culturing in CO2
\nincubator (hands-on)<\/td>Perform a 3-1 split of >70%
\nconfluent T-flasks (hands-on)<\/td><\/td>\n<\/tr>\n \n\t 3:00 PM-4:00 PM<\/td> Wrap-up, review, and looking ahead (discussion)<\/td> Wrap-up, review, and looking ahead (discussion)<\/td> <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"