Location: URI <\/strong> <\/em> This WDT Module (Drug Interactions by CYP450 Enzymes) is designed to instruct students in an important biochemical assay that is used in the pharmaceutical industry during pre-clinical drug discovery and development. Instruction will include both theoretical and hands-on practical training.<\/p>\n Participants will learn to conduct the enzyme assay used to detect drug-drug interactions through inhibition of cytochrome P450 (CYP) drug metabolizing enzymes using the widely used Vivid\u00ae CYP450 Screening Kit.<\/p>\n Students will review basic skills such as micropipetting and making solutions of known concentrations while gaining more advanced skills in fluorescence spectroscopy to measure concentration, enzyme inhibition kinetics, and drug metabolism-based drug-drug interactions.<\/p>\n The module will be completed over 2.5 days and participants who complete the module will receive an RI-INBRE Certificate of completion<\/p>\n It is recommended that students bring a tablet, iPad, or laptop to make entries in their electronic lab notebook. Lecture slides and laboratory handouts will be provided to students.<\/p>\n Introduction to Enzyme-Drug Assays Instructor: Roberta King, PhD, University of Rhode Island Location: URI Session 1: June 24 – June 26 ( Location TBD) Session 2: July 7-9 ( Location TBD) Course Overview This WDT Module (Drug Interactions by CYP450 Enzymes) is designed to instruct students in an important biochemical assay that is used […]<\/p>\n","protected":false},"author":3192,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[1],"tags":[],"class_list":["post-36060","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/36060","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/users\/3192"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/comments?post=36060"}],"version-history":[{"count":5,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/36060\/revisions"}],"predecessor-version":[{"id":37971,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/36060\/revisions\/37971"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/media?parent=36060"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/categories?post=36060"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/tags?post=36060"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nSession 1: June 24 – June 26 ( Location TBD)
\nSession 2: July 7-9 ( Location TBD)<\/p>\nCourse Overview<\/h3>\n
Learning Outcomes<\/h2>\n
\n
Lab Report<\/h2>\n
\n
Additional Information:<\/h2>\n
Proposed Timeline (subject to change)<\/h2>\n\n
\n\n
\n\t \n<\/td> Day 1<\/th> Day 2<\/th> Day 3<\/th>\n<\/tr>\n<\/thead>\n \n\t 9:00 AM-10:00 AM<\/td> Overview (Classroom)<\/td> Discuss results from previous day
\n(classroom)<\/td>CYP assay set #5<\/td>\n<\/tr>\n \n\t 10:00 AM-11:00 AM<\/td> CYP assay #1: Detect known concentrations of your metabolite (dilution series and fluorescence measurement) (Hands-on) <\/td> CYP assay set #3<\/td> CYP assay set #5 continued<\/td>\n<\/tr>\n \n\t 11:00 AM-12:00 PM<\/td> Graph data, discuss results, prepare for afternoon. (Hands-On) <\/td> Graph data, discuss results, prepare for afternoon. (Lecture and Hands-On)<\/td> Graph data, Discussion of results, Wrap up.
\n<\/td>\n<\/tr>\n\n\t 12:00 - 1:00 PM<\/td> Break<\/td> Break<\/td> WDT Survey and
\nCertificate Distribution<\/td>\n<\/tr>\n\n\t 1:00 PM-2:00 PM<\/td> CYP assay set #2 (Hands-On) <\/td> CYP assay set #4<\/td> <\/td>\n<\/tr>\n \n\t 2:00 PM-3:00 PM<\/td> CYP assay set #2 continued<\/td> CYP assay set #4 continued<\/td> <\/td>\n<\/tr>\n \n\t 3:00 PM-4:00 PM<\/td> Prepare for CYP assay #3, make own inhibitor dilution series. Graph data, Discussion of results<\/td> Graph data, Discussion of results<\/td> <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"