{"id":37917,"date":"2026-04-01T10:20:29","date_gmt":"2026-04-01T14:20:29","guid":{"rendered":"https:\/\/web.uri.edu\/riinbre\/?p=37917"},"modified":"2026-04-01T12:38:16","modified_gmt":"2026-04-01T16:38:16","slug":"wdt-2026-crispr-cas9-experiment-and-design-validation-for-biomanufacturing","status":"publish","type":"post","link":"https:\/\/web.uri.edu\/riinbre\/wdt-2026-crispr-cas9-experiment-and-design-validation-for-biomanufacturing\/","title":{"rendered":"WDT 2026 – CRISPR\/Cas9 Workshop"},"content":{"rendered":"\n

CRISPR-Cas9 Experiment Design and Validation for Biomanufacturing<\/h1>\n\n\n\n

Instructor: Kaitlin M. Dailey, PhD, URI<\/h2>\n\n\n\n

Location: URI<\/em><\/strong><\/p>\n\n\n\n

Session 1: June 15-17 (Room 303)<\/p>\n\n\n\n

Session 2: June 24-26 (Room 303)<\/p>\n\n\n\n

Course Overview<\/h3>\n\n\n\n

This workforce development workshop module was designed to strengthen Rhode Island\u2019s biomanufacturing talent pipeline by providing participants with applied, industry\u2011relevant training in CRISPR\u2011Cas9 experiment design and validation. The workshop emphasizes how genome-editing technologies are used in modern biomanufacturing workflows, including strain engineering, process optimization, quality control, and product consistency. Participants will be guided through the full experimental lifecycle, from target identification and guide RNA design to control selection, validation strategies, and data interpretation, with a strong focus on reproducibility, scalability, and regulatory awareness – core competencies required in biomanufacturing and translational research environments.<\/p>\n\n\n\n

The module highlights validation approaches critical to biomanufacturing contexts, including genotypic confirmation, phenotypic screening, off\u2011target risk assessment, and documentation practices aligned with quality systems and good laboratory\/manufacturing principles. Case studies and applied design exercises connect CRISPR decision\u2011making to downstream manufacturing considerations such as yield stability, process robustness, and technology transfer. By emphasizing experimental rigor, the workshop will equip participants with transferable skills directly relevant to roles in biotech industries.<\/p>\n\n\n\n

This workshop directly supports Rhode Island\u2019s life sciences and biomanufacturing workforce priorities by developing core biotechnical competencies. At the national level, the training aligns with federal workforce development goals to strengthen domestic biomanufacturing capacity and expand the skilled technical workforce. By connecting foundational genome-editing knowledge to real-world manufacturing applications, the workshop contributes to an innovation-ready workforce capable of supporting both near-term industry needs and long-term national bioeconomy objectives.<\/p>\n\n\n\n

Learning Outcomes<\/h3>\n\n\n\n
    \n
  • Apply CRISPR\u2011Cas9 genome editing principles to biomanufacturing\u2011relevant contexts<\/strong>\u00a0by designing gene\u2011editing experiments that account for strain engineering goals, process robustness, reproducibility, and downstream manufacturing considerations.<\/li>\n\n\n\n
  • Implement appropriate validation and quality\u2011control strategies<\/strong>\u2014including genotypic confirmation, phenotypic assessment, off\u2011target risk evaluation, and documentation practices\u2014aligned with biomanufacturing standards and quality\u2011driven laboratory and production environments.<\/li>\n\n\n\n
  • Translate genome\u2011editing design decisions into workforce\u2011ready competencies<\/strong>\u00a0by connecting experimental planning and data interpretation to scalability, regulatory awareness, and technology transfer needs across biotechnology and biomanufacturing sectors.<\/li>\n<\/ul>\n\n\n\n

    Lab Report<\/h3>\n\n\n\n

    Students will be expected to:<\/p>\n\n\n\n

      \n
    • Maintain clear, accurate, and contemporaneous laboratory notebooks that document experimental objectives, protocols, materials, conditions, deviations, and observations in sufficient detail to support reproducibility and troubleshooting.<\/li>\n\n\n\n
    • Record data and results systematically, including raw data, calculations, controls, and validation outcomes, using standardized formats and clear labeling consistent with laboratory and biomanufacturing best practices.<\/li>\n\n\n\n
    • Design and validate a genetic modification and perform the initial steps\u00a0in vitro<\/em>\u00a0to implement the schematic.<\/li>\n<\/ul>\n\n\n\n

      Resources Used in this Module<\/h3>\n\n\n\n
        \n
      • Standard Operating Procedures \u2013 DNA extraction methods, PCR, restriction digest, ligation, plasmid generation and cloning.<\/li>\n\n\n\n
      • SerialCloner software for\u00a0in silico<\/em>\u00a0experimental design and validation<\/li>\n\n\n\n
      • Microsoft Powerpoint slide decks for lectures<\/li>\n<\/ul>\n\n\n\n

        Proposed Timeline (Subject to Change)<\/h3>\n\n\n\n
        <\/td>Day 1<\/td>Day 2<\/td>Day 3<\/td><\/tr>
        8:30 AM – 9:00 AM<\/td>Welcome, safety and workforce framing<\/td>Day 2 briefing<\/td>Result synthesis and communication<\/td><\/tr>
        9:00 AM – 10:30 AM<\/td>Genetic engineering and CRISPR-Cas9 foundations<\/td>Supervised Benchwork: genetic engineering execution<\/td>Biomanufacturing and workforce alignment<\/td><\/tr>
        10:30 AM – 10:45 AM<\/td>Break<\/td>Supervised Benchwork: genetic engineering execution<\/td>Break<\/td><\/tr>
        10:45 AM – 12:15 PM<\/td>CRISPR experiment design (design thinking)<\/td>Supervised Benchwork: genetic engineering execution<\/td>Reflection, assessment and next steps. This will occur from 11:00 AM – 12:30 PM.<\/td><\/tr>
        12:15 PM – 1:00 PM<\/td>Lunch<\/td>Lunch<\/td><\/td><\/tr>
        1:00 PM – 2:30 PM<\/td>Laboratory documentation and data planning<\/td>Benchwork validation and initial analysis<\/td><\/td><\/tr>
        2:30 PM – 2:45 PM<\/td>Break<\/td>Break<\/td><\/td><\/tr>
        2:45 PM – 4:30 PM<\/td>Benchwork orientation and pre-lab activities<\/td>Data Interpretation & Quality Thinking<\/td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

        <\/p>\n","protected":false},"excerpt":{"rendered":"

        CRISPR-Cas9 Experiment Design and Validation for Biomanufacturing Instructor: Kaitlin M. Dailey, PhD, URI Location: URI Session 1: June 15-17 (Room 303) Session 2: June 24-26 (Room 303) Course Overview This workforce development workshop module was designed to strengthen Rhode Island\u2019s biomanufacturing talent pipeline by providing participants with applied, industry\u2011relevant training in CRISPR\u2011Cas9 experiment design and […]<\/p>\n","protected":false},"author":5477,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[1],"tags":[],"class_list":["post-37917","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/37917","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/users\/5477"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/comments?post=37917"}],"version-history":[{"count":4,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/37917\/revisions"}],"predecessor-version":[{"id":37925,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/posts\/37917\/revisions\/37925"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/media?parent=37917"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/categories?post=37917"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.uri.edu\/riinbre\/wp-json\/wp\/v2\/tags?post=37917"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}