{"id":38038,"date":"2020-12-07T21:57:26","date_gmt":"2020-12-08T02:57:26","guid":{"rendered":"https:\/\/web.uri.edu\/rinsfepscor\/?p=38038"},"modified":"2021-05-21T17:08:36","modified_gmt":"2021-05-21T21:08:36","slug":"investigating-the-changes-to-the-glycome-of-oxyhhris-marina-under-resource-deprivation","status":"publish","type":"post","link":"https:\/\/web.uri.edu\/rinsfepscor\/2020\/12\/07\/investigating-the-changes-to-the-glycome-of-oxyhhris-marina-under-resource-deprivation\/","title":{"rendered":"CB-12: Investigating the changes to the glycome of Oxyrrhis marina under resource deprivation"},"content":{"rendered":"<p><strong>Mentor: <a href=\"https:\/\/departments.bryant.edu\/science-and-technology\/faculty\/reid-christopher\">Christopher Reid<\/a><\/strong>, <em>Bryant University<\/em><\/p>\n<h3>Project Location<\/h3>\n<p>Bryant University<\/p>\n<h3>Project Description<\/h3>\n<figure id=\"attachment_27719\" aria-describedby=\"caption-attachment-27719\" style=\"width: 290px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-27719\" src=\"https:\/\/web.uri.edu\/wp-content\/uploads\/sites\/848\/C_Reid_WEB-290x300.jpg\" alt=\"Reid\" width=\"290\" height=\"300\"><figcaption id=\"caption-attachment-27719\" class=\"wp-caption-text\">Dr. Christopher Reid<\/figcaption><\/figure>\n<p><em>Oxyrrhis marina<\/em> is a globally distributed heterotrophic dinoflagellate commonly found in marine and saltwater coastlines. Their cell surface displays an array of polysaccharides, both N- and O-linked. These carbohydrates are used by cells for a multitude of functions including communication, adhesion, and regulation. In a previous study in our lab, changes to the <em>O. marina<\/em> neutral lipid content during starvation was observed, which ultimately has led to curiosity of starvation induced changes to the cell surface, in particular N-linked glycans.<\/p>\n<p>We will continue to use <em>O. marina<\/em> as a model to investigate the changes to the cell&#8217;s surface N-glycans under satiated and starvation conditions. <em>O. marina<\/em> will be subjected to long term resource deprivation (21 days) and the changes to the cell surface analyzed. The N-glycans were liberated via enzyme treatment, and labeled with 2-aminobenzamide (2AB) via reductive amination. Derivatized glycan profiles were analyzed by MALDI-TOF mass spectrometry. The resulting glycan profiles were then annotated using available databases (ie Glycomod) and via manual analysis. Our characterization of the <em>O. marina<\/em> surface provides opportunities for a greater understanding of cell signaling and communication in saturation and starved conditions. In addition, this work will provide the first report of the N-linked glycome in this extensively studied marine dinoflagellate.<\/p>\n<h3>Additional Information<\/h3>\n<ul>\n<li><a href=\"https:\/\/news.bryant.edu\/bryant-scientist-christopher-reid-secures-universitys-first-independent-grant-national-science\">&#8220;Bryant scientist Christopher Reid secures University\u2019s first independent grant from the National Science Foundation,&#8221;<\/a> piece from Bryant News<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>CB-12: Investigating the changes to the glycome of Oxyrrhis marina under resource deprivation<br \/>\nChristopher Reid, Bryant University<\/p>\n","protected":false},"author":1994,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[947],"tags":[129,958,952],"class_list":["post-38038","post","type-post","status-publish","format-standard","hentry","category-21-surf","tag-bryant-university","tag-flex-21-surf","tag-cb-21-surf"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts\/38038","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/users\/1994"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/comments?post=38038"}],"version-history":[{"count":5,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts\/38038\/revisions"}],"predecessor-version":[{"id":40803,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts\/38038\/revisions\/40803"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/media?parent=38038"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/categories?post=38038"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/tags?post=38038"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}