{"id":41287,"date":"2021-12-11T15:52:33","date_gmt":"2021-12-11T20:52:33","guid":{"rendered":"https:\/\/web.uri.edu\/rinsfepscor\/?p=41287"},"modified":"2021-12-11T15:52:33","modified_gmt":"2021-12-11T20:52:33","slug":"cb-8-studying-toxin-production-and-microbiomes-in-the-harmful-algal-bloom-genus-pseudo-nitzschia-through-culture-experiments-and-genetic-analysis","status":"publish","type":"post","link":"https:\/\/web.uri.edu\/rinsfepscor\/2021\/12\/11\/cb-8-studying-toxin-production-and-microbiomes-in-the-harmful-algal-bloom-genus-pseudo-nitzschia-through-culture-experiments-and-genetic-analysis\/","title":{"rendered":"CB-8: Studying toxin production and microbiomes in the Harmful Algal Bloom genus Pseudo-nitzschia through culture experiments and genetic analysis."},"content":{"rendered":"

Mentor:<\/strong> Julie Maurer (University of Rhode Island)
\nCo-Mentor(s): Bethany Jenkins (University of Rhode Island)<\/p>\n

Project Location<\/h3>\n

University of Rhode Island – Kingston<\/p>\n

Project Description<\/h3>\n

Harmful algal blooms (HABs) caused by the single-celled algae (phytoplankton) Pseudo-nitzschia<\/em> have recently begun to occur in Narragansett Bay. This phytoplankton genus produces a potent neurotoxin called domoic acid which can bioaccumulate in the food web, for example shellfish, and be consumed by humans. Consuming high concentrations of domoic acid has been associated with cases of serious illness and even death due to Amnesic Shellfish Poisoning. Domoic acid production is known to be influenced by a variety of limiting nutrients and bacterial interactions, though we don\u2019t fully understand why or how Pseudo-nitzschia<\/em> make domoic acid. This SURF project aims to study the cellular mechanisms behind domoic acid production in the genus Pseudo-nitzchia<\/em> from Narragansett Bay isolates through culture-based experiments, microbiome analysis, and DNA sequencing.<\/p>\n

Responsibilities will include: Dissecting\/compound microscopy for Pseudo-nitzschia<\/em> culture isolation and assessing culture growth, maintaining culture bank, DNA extraction and amplification, PCR, analysis of genetic data, experimental design and testing (hypothesis, control\/treatment, etc.), time management, and research notebook maintenance. The student should expect to assist with field work and field sample processing as well.<\/p>\n

This project involves:<\/h3>\n
    \n
  • field work<\/li>\n
  • lab work<\/li>\n
  • computational work<\/li>\n<\/ul>\n

    Available for SURF Flex?<\/h3>\n

    Yes<\/p>\n

    Required\/preferred skills<\/h3>\n

    Preferred qualifications include experience with aseptic laboratory technique, laboratory safety, attention to detail, basic knowledge of molecular techniques (DNA extraction, PCR), basic R skills.<\/p>\n

    No prior field experience required, though student will have opportunities to assist with offshore field work on a small boat.<\/p>\n

    2022<\/p>\n","protected":false},"excerpt":{"rendered":"

    Mentor: Julie Maurer (University of Rhode Island) Co-Mentor(s): Bethany Jenkins (University of Rhode Island) Project Location University of Rhode Island – Kingston Project Description Harmful algal blooms (HABs) caused by the single-celled algae (phytoplankton) Pseudo-nitzschia have recently begun to occur in Narragansett Bay. This phytoplankton genus produces a potent neurotoxin called domoic acid which can […]<\/p>\n","protected":false},"author":1994,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[969],"tags":[],"class_list":["post-41287","post","type-post","status-publish","format-standard","hentry","category-2022-surf-projects"],"acf":[],"_links":{"self":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts\/41287","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/users\/1994"}],"replies":[{"embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/comments?post=41287"}],"version-history":[{"count":1,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts\/41287\/revisions"}],"predecessor-version":[{"id":41288,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/posts\/41287\/revisions\/41288"}],"wp:attachment":[{"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/media?parent=41287"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/categories?post=41287"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.uri.edu\/rinsfepscor\/wp-json\/wp\/v2\/tags?post=41287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}