Cell Harvesting

Participants will learn about the theories and practices of initial cell harvest and separation

Critical parameters and performance indicators provide confirmation of technicians’ proficiencies in cell harvest and initial cell separation methodologies.

Participants gain knowledge of the theories and practices of initial cell harvest and separation. Critical parameters and performance indicators provide confirmation of technicians’ proficiencies in cell harvest and initial cell separation methodologies. 

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Objectives

  • Attain knowledge about the theories and practices associated with initial cell harvest and separation techniques
  • Perform hands-on techniques associated with acid precipitation of suspension cell culture, centrifugation and harvest of supernatant, and depth filtration of supernatant and preparation for downstream processing.
  • Understand critical process parameters and performance indicators required for optimized cell harvest (yield and retention) and cell separation.

 

Who should attend this course?

Course work and laboratory exercises are designed for individuals working in the biomanufacturing industry including roles such as Upstream and Downstream Technicians, Quality Assurance, Quality Control, Bioprocess Engineers, Facilities, and Plant Operations. 

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Delivery

In-person lectures and hands on activities in a simulated GMP facility.        

  • Duration: 1 Day
  • Time: 8:00am to 4:00pm
  • Location: Kingston, RI
  • Max Number of Participants: 15

 

Instructors

Content Outline

Lecture

    1. Cell harvest Overview
      • What is cell separation?
      • Batch and Perfusion/continuous processes
    2. Methods for separation
      • Precipitation
      • Theory supporting precipitation methods
      • Modes of precipitation
      • Yield and Retention
      • Process Parameters and Performance Indicators
  1. Centrifugation
    • Theory supporting centrifugation methods
    • Types of centrifuges
    • Yield and Retention
    • Process Parameters and Performance Indicators
  2. Filtration
    • Theory supporting filtration methods membranes and Types of filtration methods
    • Yield and Retention
    • Process Parameters and Performance Indicators
  3. Systems Summary and Challenges

Lab Exercises

  1. Acid precipitation of suspension cell culture
  2. Centrifugation of cell culture and harvest of supernatant
  3. Filtration of supernatant and preparation for downstream processes
  4. Analysis of filtrate for desired cell product