Instruments in Action
Dr. Fang Wang (RI-INBRE investigator, SURF mentor, and Assistant Professor in the URI Chemistry Department) published two notable papers on protein and 
peptide synthetic modification attaining crucial data which included high-resolution mass spectra and MS/MS analyses, using the AB SCIEX .
TripleTOF 4600 mass spectrometer equipped with a DuoSpray™ ion source instrument located in the CRCF and facilitated by the CRCF.The first paper was featured in Bioconjugate Chemistry and the second paper published in the Journal of the American Chemical Society (JACS). These two papers highlight a novel strategy distinctive from conventional cysteine bioconjugation methods. Using highly reactive and hydrophilic pyridinium salts, quantitative cysteine bioconjugation can be achieved at low micromolar concentrations under fully biocompatible conditions. The versatility of this chemistry is underscored by its ability to accommodate various functionalities and synthetic handles useful for chemical biology without a significant loss in reactivity. Overall, the authors anticipate this chemistry to be a practical and user-friendly addition to the chemical biology toolbox.
