Principles of Drug Delivery Formulations
Instructor: Jyothi Menon, PhD, URI, and Kalindu Perera, MS, URI
Location: URI
Session 1: July 8 – July 10 ( Lab: Room 305 / Lecture Room: Avedisian Rm 207)
Session 2: July 17 – July 19 ( Lab: Room 305 / Lecture Room: Avedisian Rm 205)
Course Overview
This WDT Module aims to train students on the development and characterization of next-generation drug delivery platforms such as nanoparticles, microparticles and hydrogels. The module will include introductory lectures on drug delivery systems, hands-on laboratory experience with nano/microparticle and hydrogel synthesis and their characterization using techniques like dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and fluorescence microscopy. This module is intended to provide theoretical and lab-based instruction and training on drug delivery systems, and data generation and analysis, to prepare them for further training in these and related research areas. The module will be completed over 2.5 days and participants who complete the module will receive an RI-INBRE Certificate of completion.
Learning Outcomes
At the end of this module, students will be able to:
- Describe the fundamental principles of drug delivery technologies and their
applications. - Synthesize polymeric hydrogels, microparticles and nanoparticles
- Operate the DLS, FTIR and UV-Vis spectrometer and analyze the data obtained
- Collect and process fluorescence images obtained using an EVOS M5000 fluorescence microscope
- Communicate their findings in a verbal format using their own data and data presented in published scientific literature
Lab Report
Students will be expected to maintain detailed laboratory notes to include:
- Nanoparticle/ Microparticle/ Hydrogel synthesis protocols
- Protocols for operating the different instruments (DLS, EVOS, FTIR)
- Data analysis protocols (e.g., workflows, software used, etc.)
- Observations during the experiments and final data/ graphs generated
Resources Used in this Module:
- Standard Operating Procedure – Nanoparticle synthesis (available in the lab)
- Trainings to be completed prior to starting the module:
- Working Safely with Nanomaterials
- Bloodborne Pathogens & Biosafety Training
Timeline
| Day 1 | Day 2 | Day 3 | |
|---|---|---|---|
| 9:00 AM-10:00 AM | Introduction to drug delivery, basic concepts/ principles (Lecture) | Medical applications of drug delivery formulations (Lecture) | Nanoparticle cytotoxicity testing in vitro (demo + hands-on) |
| 10:00 AM-11:00 AM | Tour of the laboratory and RIN2 facility | Drug release analysis and practice calculations (Lecture); Sample collection for drug release (continued, hands-on) | Gelatin hydrogel synthesis (Hands-On); Group work on presentations |
| 11:00 AM-12:00 PM | Drug delivery formulation synthesis and characterization (Lecture) | Polymer nanoparticle synthesis; Introduction to using DLS (Hands-on) | Group presentations (groups of 2 or 3) |
| 12:00 - 1:00 PM | Break | Break | WDT Survey and Certificate Distribution |
| 1:00 PM-2:00 PM | Synthesis of alginate microparticles (Hands-On) | DLS (contd.; hands-on); Nanoparticle treatment of cells for cytotoxicity testing (Demo) | |
| 2:00 PM-3:00 PM | Synthesis of PLGA microparticles; Sample collection for drug release (Hands-on) | Final timepoint for microparticle drug release; Drug release data collection and analysis (Hands-On) | |
| 3:00 PM-4:00 PM | Particle imaging using EVOS microscopy; Sample collection for drug release (Lecture/ Hands-on) | FTIR data collection and analysis (Hands-on) |