RI-INBRE Acquires Advanced Instrumentation for Single Cell and Spatial Omics Research
We have completed the purchase of new 10X Genomics Chromium X single-cell omics system and a 10X Genomics Visium CytAssist instruments which are housed in the CRCF. These instruments will allow our cores to offer services in single-cell omics and discovery-level spatial omics. This is a significant upgrade to the ability of RI-INBRE and affiliated researchers to conduct cutting edge omics-based analyses. We are currently training core personnel on the use of these instruments and will provide training to the broader community in the coming weeks.
10X Genomics Chromium X for Single Cell Assays. The Chromium X is a 10X Genomics next-generation single-cell system, allowing for low-to-high
throughput single-cell assays using the latest 10X Genomics chemistries and analysis of millions of cells. This instrument allows users to conduct assays for gene expression (RNA-seq), chromatin accessibility (ATAC-seq), multiomic ATAC + gene expression, and single-cell immune profiling. You can find more information on the Chromium X at 10X Genomics’ site (Link).
10X Genomics Visium CytAssist for Discovery Spatial Omics Assays. The
10X Visium HD platform allows for discovery spatial omics. Using standard tissue slice methods, users can perform gene or protein expression analyses on the same samples used for microscopy applications. When combined with single-cell data from the Chromium X, users will be able to study gene expression at single-cell resolution in a spatial context, which can then be used to inform more advanced in situ spatial analyses. The Visium CytAssist instrument simplifies the process of transferring tissue samples to Visium slides. You can find more information on the Visium platform and the CytAssist at 10X Genomics’ site (Link).
We are very interested in potential use cases of these instruments and in hearing from the user community about what types of experiments they would like to run. Please feel free to contact us at any time for more information (Dr. Ang Cai (CRCF Director, angcai@uri.edu), Janet Atoyan (CRCF Lab Manager,jatoyan@uri.edu ), Dr. Chris Hemme (MIC Director, hemmecl@uri.edu )).