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Rhode Island IDeA Network of Biomedical Research Excellence

(RI-INBRE)

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  3. Rhode Island INBRE Molecular Informatics Core (MIC)
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Robotic Sample Preparation

Wafergen Apollo 324 NGS Library Preparation System

The Apollo 324 is a benchtop system that automates next-generation sequence (NGS) library preparation workflows by using bead technology to execute high-performance isolation and purification of nucleic acids (DNA, RNA, plasmid and so on) and proteins.  The system automates end-repair, A-tail addition, adapter ligation and final double-bead-based cleanup and size selection.  The Apollo 324 enables simultaneous processing of one to eight samples, with batch processing for 32 samples at a time.  Up to eight samples can be prepared in approximately four hours, yielding products ready for amplification or direct sequencing, and a batch of 32 libraries can be created in less than eight hours.

Specifications:

  • Samples per run: 1–8 or 32
  • Throughput/day: 16 samples: 2 runs of 8 samples | 32 samples: 1 run of 32 samples
  • Run time: 1–8 samples: ~ 3.5 hours | 32 samples: ~ 7 hours
  • Setup time: 1–8 samples: ~ 15 minutes | 32 samples: ~ 1 hour
  • Temperature control: 2 Peltier-type blocks (4–95 °C), PID regulated
  • Magnetic capture: In-tip capture
  • Plate format: 96-well, SBS conforming

 

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