Proteomics & Genomics

Protein Sequencer

PPSQ31AThe Shimadzu PPSQ-31A protein sequencer uses the Edman degradation reaction method.  The sequencer separates PTH-Amino acids isocratically providing more stable retention times. Analysis in isocratic mode also allows for eluents to be recycled, thereby reducing the cost and disposal of the mobile phase.   The 31A has one reactor and the cycle time is 47min/cycle.  Glass fiber disks or PVDF membrane may be used for sample immobilization.

 

DNA/RNA synthesizer

MM4.Machine.Computer-cropThe MerMade-4 Oligonucleotide synthesizer is designed to synthesize DNA, RNA & LNA oligonucleotides in a column format using standard or modified chemistries. The machine comes with a 4 column configuration and is capable of making a combination of standard, degenerate, and modified oligos in the same run with scales varying from 50nmole to 5umole. A typical run time is less than 2 hours for 4 columns of 20mers.

 

2d electrophoresis system

PROTEAN IEF System for 2-D with Immobilized pH Gradients

Protean IEF 2The PROTEAN IEF system, consisting of ReadyStrip IPG strips and the PROTEAN IEF cell, is optimized to perform first-dimension isoelectric focusing — simply, reproducibly, and efficiently. Choose strips in four lengths, each designed to match Bio-Rad’s second-dimension cells. Control resolution with IPG gradients that offer 1, 3, or 7 pH units per strip.

 

 

 

Real-Time PCR

The ViiA7 Real-Time PCR System from Life Technologies provides enhanced fluorescence detection with the OptiFlex System. The ViiA7 offers simplified workflow, intuitive software, touch screen interface, and one-button protocols for error minimization.

pcr

 

 

 

 

 

 

 

offgel fractionator

Offgel fractionatorThe Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pl-based fractionation.  The resulting fractions are in solution, making recovery for LC/MS analysis much easier than with old-fashioned gels.

Features of OFFGEL Fractionator

  • pl-based OFFGEL fractionation with 0.1 – 0.6 pH resolution depending on consumable kit used
  • In-solution fraction recovery for easy transfer to LC/MS
  • Two power supplies allow simultaneous fractionation of two sample sets with broad differences in concentration
  • Up to 16 samples fractionated at the same time
  • µg to mg load capacity
  • Possible to run conventional in-gel IEF as well as OFFGEL fractionation
  • Preconfigured methods for OFFGEL and in-gel modes