Investigator: Amanda Jamieson, Brown University

Mentor: Jack Elias, Brown University

Scientific Theme: Molecular Toxicology and Cancer

Abstract: While the effect of tobacco smoke on the lung is well documented, virtually nothing is known about the molecular toxicology and cancer risk of e-cigarettes. These devices are becoming increasingly popular, but the long-term impact on public health has not been determined. This proposal will explore the impact of e-cigarette vapor on cells of the pulmonary system. Using in vitro systems of lung epithelial, endothelial, and innate immune cells we will determine the toxic effects of chemicals from e-cigarettes (Aim1). In this Aim we will explore the direct effects that e-cigarette vapor has on causing stress and damage to the airway epithelium and endothelium. Macrophages have important functions in the immune response, the anti- inflammatory response, and in tissue repair. Therefore, determining how e-cigarette vapor alters macrophage development and function will allow us to understand how e-cigarette exposure may alter an important regulatory cell of the lung. We will also use an in vivo animal model of e-cigarette vapor exposure to determine alterations of lung physiology, increased lung damage and increased cancer risk (Aim 2). These studies will be pioneering studies in examining this new technology. Determining the toxicology of e-cigarette vapor on lungs has important public health indications.

Human Health Relevance: E-cigarette use is increasing in popularity, but very few studies have been done to evaluate the safety and long-term consequences of use. This proposal will determine the impact of e-cigarette vapor on the pulmonary innate immune cells, pulmonary epithelial cells, and lung endothelial microvasculature. In addition an in vivo animal model will determine the risk of e-cigarette vapor on lung cancer risk.