RI Genomics & Sequencing Center

Services include DNA sequencing and fragment analysis (Sanger method), next generation sequencing and library preparation (Illumina MiSeq), light and scanning confocal microscopy, and genomics equipment use (qPCR, BioAnalyzer, Nanodrop, etc.).

  • AirClean 600 PCR Workstation – combines an ISO 5 / Class 100 clean air environment with UV light sterilization to provide optimal protection from sample contamination.
  • Agilent 2100 BioAnalyzer
 – microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells.
  • Applied Biosystems 3130xl genetic analyzer
 – fluorescence-based analysis system used for DNA sequencing and fragment analysis.
  • Beckman Coulter Allegra 25R – refrigerated centrifuge.
  • Covaris S220 AFA ultrasonicator – used to shear or fragment DNA samples for the preparation of sample libraries for analysis in next generation sequencers.
  • DNAStar Lasergene server
 – comprehensive suite of software for DNA & protein sequence analysis, contig assembly and sequencing project management
  • Eppendorf epMotion 5075 VAC  – automated liquid handling workstation for diverse methods such as nucleic acid purification, PCR setup, cleanup of sequencing reactions, etc.
  • Eppendorf Mastercycler ep Gradient S thermal cycler
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  • Illumina MiSeq next generation sequencer – used for a wide range of genetic analyses including whole-genome sequencing, mRNA sequencing, de novo sequencing, ChIP-Seq, targeted resequencing, and metagenomics (16S rRNA sequencing).
  • IntegenX Apollo 324 – automated workstation for next generation sequence library preparation using bead technology to execute high-performance isolation and purification of nucleic acids (DNA, RNA, plasmids, etc.) and proteins.
  • Invitrogen Qubit fluorometer – provides highly accurate quantification of DNA, RNA and protein samples.
  • MJ Research PTC-100 thermal cycler
  • Nanodrop 8000 (8-channel spectrophotometer)
 – measures concentration and purity of DNA, RNA and protein samples.
  • OmniLog Phenotype Microarray System
 – used to identify bacterial species and determine their phenotypic behavior on a wide variety of substrates and compounds.
  • Qiagen TissueLyser
 – provides rapid and efficient disruption of cells and tissues.
  • Qiagen QiaCube – automated workstation to process Qiagen spin columns for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins
  • Roche LC480 qPCR instruments (2)
 – plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids and genotyping.
  • Stratagene Mx3005 qPCR system
 – used to quantify nucleic acids for studying gene expression, mutational analysis, disease state, and gene dosage.
  • Sage Science BluePippin – a preparative electrophoresis platform that separates, sizes and extracts DNA fragments.
  • Thermo Savant DNA SpeedVac
 – a centrifugal concentration system for drying low-volume ethanol or isopropanol precipitates of DNA and RNA.
  • Vibratome 5000 cryostat – uses a microtome housed inside a chamber with a temperature of -35°C for the preparation of frozen sections of cells and tissues.
  • Zeiss AxioImager 2 motorized microscope with AxioCam high-resolution digital camera and
 LSM 700 scanning confocal module with 4 lasers at 405, 488, 555, & 639 nm excitation