RI-INBRE Workforce Development Training (WDT) Summer 2023 Syllabus

Module Title: Advanced Drug Delivery Formulations

Instructor: Jyothi Menon, PhD, and Andrea Gonsalves, MS

Course Overview

This WDT Module aims to train students on the development and characterization of next-generation drug delivery platforms such as nanoparticles, microparticles and hydrogels. The module will include introductory lectures on drug delivery systems, hands-on laboratory experience with nano/microparticle and hydrogel synthesis and their characterization using techniques like dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and fluorescence microscopy. This module is intended to provide theoretical and lab-based instruction and training on drug delivery systems, and data generation and analysis, to prepare the students for further training in these and related research areas. The module will be completed
over 2.5 days and participants who complete the module will receive an RI-INBRE Certificate of Completion.

Learning Outcomes

At the end of this module, students will be able to:

  • Describe the fundamental principles of drug delivery technologies and their
  • Synthesize polymeric hydrogels, microparticles and nanoparticles.
  • Operate the DLS, FTIR and UV-Vis spectrometer and analyze the data obtained.
  • Collect and process fluorescence images obtained using an EVOS M5000 fluorescence microscope.
  • Communicate their findings in a verbal format using their own data and data presented in published scientific literature.

Lab Report

Students will be expected to maintain detailed laboratory notes to include:

  • Nanoparticle/ Microparticle/ Hydrogel synthesis protocols
  • Protocols for operating the different instruments (DLS, EVOS, FTIR).
  • Data analysis protocols (e.g., workflows, software used, etc.)
  • Observations during the experiments and final data/ graphs generated.

Resources Used in this Module:

  • Standard Operating Procedure – Nanoparticle synthesis (available in the lab)
  • Trainings to be completed prior to starting the module:
  • Working Safely with Nanomaterials
  • Bloodborne Pathogens & Biosafety Training


 Day 1Day 2Day 3
9:00 AM-10:00 AMIntroduction to drug
delivery, basic
concepts/ principles (Lecture)
Medical applications of drug delivery formulations (Lecture)Nanoparticle cytotoxicity
testing in vitro (demo + hands-on)
10:00 AM-11:00 AMTour of the laboratory
and RIN2 facility
Drug release analysis and
practice calculations
Sample collection for drug release (continued, Hands-On)
Hydrogel synthesis (Hands-on); group work on presentations
11:00 AM-12:00 PMDrug delivery
formulation synthesis
and characterization (Lecture)
Polymer Nanoparticle
synthesis (Hands-on)
Group presentations (groups of 2 or 3)
12:00 - 1:00 PMBreakBreakClosing Group Lunch
1:00 PM-2:00 PMSynthesis of alginate
microparticles (Hands-
Introduction to using DLS
2:00 PM-3:00 PMSynthesis of PLGA
Final timepoint for
microparticle drug release;
drug release data collection and analysis (Hands-on)
3:00 PM-4:00 PMParticle imaging using
EVOS microscopy;
Sample collection for
drug release (Lecture/
FTIR analysis of samples (hands-on)