Mentor: Roxanna Smolowitz, Roger Williams University
Co-Mentor: Abigail Scro, Roger Williams University

Project Location

Roger Williams University

Project Description

The RWU Aquatic Diagnostic Laboratory provides diagnostic services to the aquaculture community. Agents responsible for these diseases are commonly associated with changing environments, especially increased temperatures of the marine waters, so identifying and understanding that relationship is important. Additionally, effects of changes in the marine environment effect populations of commercially important species. As such, the student selected for this position will learn many different procedures used in the molecular identification of agents that can cause disease both in oysters, clams and humans. Method include collecting samples, DNA extraction and quantification and use of PCR and qPCR methods of agent detection. Additionally, the laboratory will be involved in three major projects this summer.

First, the ADL will be developing a new qPCR method for the detection of hemic neoplasia in hard clams (Mercenaria mercenaria), a needed diagnostic test. Second, we will work with a co-PI studying Vibrio sp. occurrence on sea weed and will identify Vibrio sp. abundance and pathogenic gene occurrence in associated oysters and on the sea weed collected as part of that study using both molecular (qPCR) methods, Most Probably Number and bacterial culture methods. Thirdly, we will study the gonadal tissues of whelks using microscopic techniques on paraffin sections to identify the reproductive cycle of whelks in RI waters. This information will be used to help establish fishing regulations in the changing environment. The student will participate in all phases of disease diagnosis and these research objectives, but will choose one of these research areas for a specific project.

Additional Information

• The Aquatic Diagnostic Lab at RWU’s Center for Economic and Environmental Development