Research

Our research includes plant transformation technology development, genome engineering, and functional genomics, with applications in crop improvement, stress resilience, and metabolic pathway engineering. We bridge fundamental discovery and translational outcomes through robust tissue culture pipelines and molecular validation.

Focus Areas

Plant Transformation and Plant Regeneration

Multi-species protocols; Maize, rice sorghum and switchgrass.- callus induction; somatic embryogenesis; rooting and acclimatization.

Genome Editing and Trait Engineering

CRISPR–Cas systems (Cas9, Cas12a),
base/prime editing, multiplex gRNA design, RNP & plasmid delivery.

Functional Genomics

Gene knockout/knock-in; expression and promoter analysis; pathway dissection.

Molecular Characterization

DNA/RNA extraction, PCR/qPCR/RT-PCR, imaging and phenotyping.

Growth and Phenotyping

Greenhouse and Controlled environments

Methods and Platforms

Agrobacterium-mediated transformation

  • Sorghum
  • Maize
  • Rice

Biolistic gene delivery (PDS-1000/He, Bio-Rad)

Microscopy -Brightfield, GFP fluorescence (FLM), Scanning electron microscopy (SEM)

Outcomes & Impact

Bioenergy and Crop Trait Development (yield, nutrition, stress tolerance)

Synthetic biology and biomanufacturing in plants

Marker-free precision breeding