Agilent 2100 Bioanalyzer

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. This Lab-on-a-Chip technology utilizes a network of channels and wells that are etched onto a glass or polymer chip. Pressure or electrokinetic forces move pico-liter volumes in a finely controlled manner through the channels. The main advantages of Lab-on-a-Chip are ease-of-use, speed of analysis, low sample and reagent consumption and high reproducibility due to standardization and automation. Results are delivered within 30-40 minutes in high quality digital data and can be shown in gel-like image, electropherogram and tabular formats.

Features of DNA chips (DNA 1000, DNA 7500, & DNA 12000 kits):

  • Minimal sample consumption – only 1 µL of sample required per analysis; 12 samples can be analyzed in each chip.
  • Sizing accuracy – normalization to two internal markers and ladders.
  • Quantification accuracy and reproducibility – automated quantification of each DNA fragment against internal standards.
  • High resolution of a large number of bands – critical for any multiplex PCR application.
  • Broad linear dynamic range – enables the detection of less abundant products, e.g. low abundance messages in multiplex RT-PCR amplifications or non-specific amplifications.
  • Sensitivity – Laser Induced Fluorescence (LIF) detection for fragments down to 0.1 ng.
  • Ready-to-use – standardized assays and pre-packaged reagent kits.

Features of RNA chips (Small RNA; RNA 6000 Pico and Nano kits):

  • Minimal sample consumption – only 1 µL of sample required per analysis or as little as 5 ng of total RNA or 50 pg of purified miRNA.
  • Fast results – analyze 11 samples in 30 minutes.
  • RNA Integrity Number (RIN) -a reliable tool to compare integrity of RNA samples automatically plus freely accessible.
  • RNA Integrity Database (RINdb) of RNA profiles.
  • Qualitative size analysis of ss- and ds-oligonucleotides – specified within a range of 6 -150 nt, based on an RNA ladder.
  • Ready-to-use – standardized assays and pre-packaged reagent kits.