Quantitative PCR

Fee* for use of the Agilent (Stratagene) Mx3005P or the Roche LightCycler 480 qPCR Systems:   $10.00 / run

*Rate charged to URI, RI-INBRE & RI-EPSCoR users.  Financial assistance has been provided by RI-EPSCoR (NSF Award EPS-1004057) and the College of the Environment and Life Sciences.

Please see our Fees page for instructions regarding payment for services.

User supplied consumables:

  • Primers 
  • DNA or RNA template 
  • Reagent kit (for qPCR, SYBR® Green protocols or any custom designed fluorescent probe such as TaqMan® or other dual-labeled probe, Scorpions, and/or Molecular Beacons) 
  • Agilent (Stratagene) optical plates/tubes and caps 
  • Roche clear or white multiwell plates and sealing film

The RIGSC has a limited supply of Mx3005P optical plates, tubes and caps and Roche multiwell plates and seals that can be used for preliminary tests.

New users of qPCR are encouraged to follow the Standard Operating Procedures for this method that are listed under the Procedures menu. These methods work well and have been used repeatedly with excellent results.

To ensure consistent experimental results between the two qPCR systems, we recommend that users follow the standardized MIQE nomenclature guidelines for publication of quantitative real-time PCR experiments: Bustin, S.A. et al. 2009. “The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments.” Clinical Chemistry 55(4) 611-622.

To reserve time on one of the qPCR instruments, please use the RIGSC Calendar. If you have any questions or problems, call the RIGSC manager at 874-5919.

Agilent’s “Introduction to Quantitative PCR – Methods and Application Guide” is an excellent introduction to qPCR.

Please contact the RIGSC for information on the current discounted pricing for reagent kits and consumables from Agilent and Roche.