- J.Christopher Octeau, Jeremy Celver, Meenakshi Sharma, Joseph Schrader, Christopher Colvin, Christopher Aiudi, Kevin Sun, Ching-Kang Chen*, Abraham Kovoor;
Research
G beta 5 (Gβ5) is a unique G protein β subunit that is thought to be expressed in vivo as an obligate heterodimer with Regulator of G protein Signaling (RGS) proteins of the R7 family. Here we report that Gbeta5 can also functionally interact with D2‑dopamine receptors (D2R). We show that Gβ5 is recruited to biochemical fractions containing D2‑dopamine receptors (D2R) and D2R localizes Gβ5 to the plasma membrane and this interaction has multiple consequences on cellular functions of D2R. D2R that is coexpressed with Gβ5 functions differently than isolated receptors. Previously we have shown that D2R predominantly exists in functional detergent insoluble membrane microdomains (Celever et al., 2012 and Sharma et al., 2013). Together, these findings represent a new cellular role for Gβ5 in modulating the signaling of GPCRs in membrane microdomains that lack
R7RGS proteins.
Related People: Abraham Kovoor
University of Rhode Island, Kingston, RI;
*Virginia Commonwealth University School of Medicine, Richmond, VA