- Presented at the 53rd Annual Interscience Conference on Antimicrobial Agents and Chemotherapy in 2013
- Megan Luther 1,2 Emily T. Martin3 , Ann Maame Araba Sam2 , Jeffrey Coleman4,5 , Eleftherios Mylonakis 4,5 and Kerry L. LaPlante 1,2,5
Research
BACKGROUND Our intent was to quantify biofilm production among Staphylococcus aureus isolates from different sources: blood, catheter, nares, tissue, and urine. We also used two molecular based techniques, ulti-locus sequence typing (MLST) and spa typing, for distinguishing between strains of S. aureus, as specific strains of S. aureus (ST8) have previously been shown to be associated with biofilm formation. We used these methods to identify trends in source, biofilm, sequence type, or spa type of methicillin-resistant S. aureus (MRSA) isolates from the Providence VAMC.
METHODS Biofilm production of 94 MRSA isolates from blood, catheter, nares, tissue, and urine from the Providence VAMC was quantified by the crystal violet biofilm method. MLST analysis was conducted using PCR. PCR was also used to determine spa type.
Related People: Kerry L. LaPlante
1Veterans Affairs Medical Center, Providence, RI;
4 Rhode Island Hospital, Providence, RI;
2 University of Rhode Island, College of Pharmacy, Kingston, RI;
3 Wayne State University, Detroit, MI;
5 Warren Alpert Medical School of Brown University, Providence, RI.