The Viral Immunity and Pathogenesis Laboratory is a ~2,400 sq ft BSL-2 laboratory with dedicated tissue culture space and molecular biology space. Major equipment includes:
- Class IIA biosafety cabinets (5)
- CO2 incubators (5; one equipped with refrigeration for 28°C culture)
- Refrigerated centrifuges (2)
- Liquid nitrogen storage tanks (6; combined capacity >100,000 vials)
- Ultralow temperature freezers (5; combined capacity >100,000 vials)
- Inverted microscope
- EVOSfl fluorescence microscope (includes GFP, RFP, DAPI, and Cy5 cubes, 100× oil immersion objective)
- Nexcelom Cellometer T4 and Mini automated cell counters
- PCR hood
- QIAcube (automated nucleic acid isolation)
- QIAgility (liquid handler/automated PCR assay setup)
- Bio-Rad dual 48-well PCR machine
- Bio-Rad CFX96 real-time PCR machine
- Bio-Rad Chemi-Doc XRS imager
- Envision multilabel plate reader (includes filters for fluorescence, luminescence, and AlphaScreen)
- BioTek Cytation3 cell imaging plate reader
- Bio-Rad Bioplex 200 Luminex
- MACSQuant-10 flow cytometer (3 lasers, 8-color capacity)
- BD LSRII flow cytometer (4 lasers, 12-color capacity)
- CTL S5 UV ELISpot reader and analyzer
- Miltenyi AutoMACS
- 10x Genomics Chromium Controller